Commander / Captain Benjamin Lafayette Sisko | Avery Brooks |  |
| Major/Colonel Kira Nerys | Nana Visitor |  |
| Odo | René Auberjonois |  |
| Dr. Julian Subatoi Bashir | Alexander Siddig |  |
| Lieutenant Commander Worf (Staffel 4-7) | Michael Dorn |  |
Lieutenant Commander Jadzia Dax (Staffel 1-6) | Terry Farrell |  |
| Chief Miles Edward O’Brien | Colm Meaney |  |
| Quark | Armin Shimerman |  |
| Jake Sisko | Cirroc Lofton |  |
Lieutenant Ezri Dax (Staffel 7) | Nicole de Boer |  |